Monitoring of Leuconostoc population during sauerkraut fermentation by quantitative real-time polymerase chain reaction.

نویسندگان

  • So-Young Kim
  • Ki-Seon Yoo
  • Yujin Kim
  • Eunyoung Seo
  • Beom Soo Kim
  • Nam Soo Han
چکیده

A real-time PCR assay method was established to monitor Leuconostoc spp. populations via specific amplification of the dextransucrase gene. Quantification of L. mesenteroides B-512F using both genomic DNA and cell suspensions yielded a log-linear correlation spanning approximately 5 log units. By using this method, monitoring changes of Leuconostoc spp. during sauerkraut fermentation was successfully accomplished with accuracy after inoculation of starter and sugars (sucrose and maltose).

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منابع مشابه

Identification and characterization of Leuconostoc fallax strains isolated from an industrial sauerkraut fermentation.

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Effects of Leuconostoc mesenteroides starter culture on fermentation of cabbage with reduced salt concentrations.

Sauerkraut fermentations rely upon selection of naturally occurring lactic acid bacteria by addition of 2.0% to 2.25% granulated sodium chloride (NaCl) to shredded cabbage. Excess brine generated is a waste product with high levels of organic material (BOD) and nonbiodegradable NaCl. The objective was to determine whether addition of Leuconostoc mesenteroides starter culture to reduced-salt cab...

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عنوان ژورنال:
  • Journal of microbiology and biotechnology

دوره 21 10  شماره 

صفحات  -

تاریخ انتشار 2011